Biopsy Submission Form (PDF)
Samples can be shipped to:
- HCS (for quicker processing)
PO Box 770
Everson, WA 98247
207 N Harkness St
Everson, WA 98247
- Barry Rickman (please email or call 425-616-8407 for details)
- Place biopsy specimen or necropsy in a plastic screw top container containing 10% Neutral Buffered Formalin. We can provide you with formalin containers upon request. Please do not send fresh or frozen, unfixed tissue.
- Small specimens and endoscopic biopsies can be placed directly in a tissue cassette. If labeling is needed, label the cassette in pencil with the anatomic site of biopsy (we can provide this too if you don't have it) and then place in the formalin jar.
- Label the Screw Top Container with Animal's name/case number, species, and tissue type. Also include your name and hospital/institute name.
- Double seal the formalin jar by placing the specimen container in a ziplock or sealable bag.
- Formalin preserves the best when the ratio of Formalin to tissue is 10:1 to 20:1.
- This guideline should help you to choose the most appropriate container size for your specimen.
- Don't forget to submit with a submission form.
Surgical Biopsy Second Opinion
Please have the laboratory email or call to coordinate obtaining slides for a second opinion.
Necropsy in a Bottle
- As with the biopsy, place multiple organs from the necropsy in a jar with 10% neutral buffered formalin at a 10:1 ratio.
- If a specific tissue site is needed to be labeled or if there is a specific tissue of extreme interest, either place in a cassette and label it with pencil or place and label in a separate jar.
- Small animals can be placed whole in formalin after opening body cavities, which include the thoracic cavity, peritoneum, coelomic cavity and the calvarium.
- Fish are particularly prone to decomposing faster than other types of animals.
- Open the visceral cavity of the fish by cutting an opening in the abdominal wall.
- It is critical to place fish in formalin immediately after euthanasia or death. Post-mortem autolytic changes can occur as early as 10 minutes post mortem.
- A 10% neutral buffered formalin fixative is required for freshwater fish and a 10% formalin in seawater for marine fish.
- We can coordinate virology testing with you if and when needed.
- All microbiology samples should be sent to a trusted laboratory that you work with for culture.
- Please contact us for a list of microbiology labs if you do not have one.
- Save some of the tissue frozen if you suspect a toxin or infectious agent. Call our lab to further discuss your case.
An MSDS (Material Data Safety Sheet), that provides health information, as well as, information regarding the manufacturer and emergency treatment is available upon request.